HPLC mobile phase

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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Hi.

I am trying to conduct individual phenolic compounds profiling using HPLC. In the methods that I searched, 0.5% acetic acid is used as mobile phase along with methanol. I found about the function of acetic acid added in mobile phase. However, I wonder the differences between glacial acetic acid and acetic acid? I've searched online for these two and found that there is not much distinction, except glacial acetic acid solidify at temperature lower than room temp. Still I wonder, will it be difference if any of these being used as mobile phase in HPLC? Can any of both being used as the mobile phase for HPLC-DAD?

I'm in the middle of ordering chemicals for my analysis. I really hope someone could help me with this.

Thank you in advance.
The term "glacial" is an old-fashioned way of indicating that the acetic acid is pure (undiluted), at which point it indeed becomes solid slightly below room temperature. Glacial acetic acid and acetic acid are the same chemicals. In any case the purity should be on the bottle that you order, which you use to calculate how much you need for a 0.5% solution.

To be sure you're buying the right purity, you can order "hplc grade-acetic acid", either as a solution or pure (=glacial).
Hi Rndirk...

Thank u very much for ur response..
So it means that both glacial acetic acid or acetic acid will do as mobile phase for HPLC as long as it is HPLC-grade ya?
Yes, it's the same thing
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