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- Posts: 2
- Joined: Thu Aug 30, 2018 6:10 am
I am trying to conduct individual phenolic compounds profiling using HPLC. In the methods that I searched, 0.5% acetic acid is used as mobile phase along with methanol. I found about the function of acetic acid added in mobile phase. However, I wonder the differences between glacial acetic acid and acetic acid? I've searched online for these two and found that there is not much distinction, except glacial acetic acid solidify at temperature lower than room temp. Still I wonder, will it be difference if any of these being used as mobile phase in HPLC? Can any of both being used as the mobile phase for HPLC-DAD?
I'm in the middle of ordering chemicals for my analysis. I really hope someone could help me with this.
Thank you in advance.