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- Posts: 76
- Joined: Tue Oct 05, 2004 10:41 am
- Location: Lugo, Spain
I am trying to set up a method for acrylamide in potato chips analysis but I don't known what is going wrong because I don't have much success. My idea is to detect acrilamide in potato chip at 100 ng/g and conduct the quantification with matrix-matched calibration curve. I have tried diferent HPLC methods with C18 columns, however, my feeling is that the acrilamide is not retained by the column and the different Rts indicated depend on the length of the peak tube. I finally manage to obtain a more o less gaussin peak using a zorbax C8 column.
On the other hand I tried to extract acrilamide with water, hot water, quechers, and SPE but the reproducibility is not very good, my RSD is about 60% for 500 ng/g.
Does anybody known a method that it is realiable and reproducible at 100 ng/g level?
Thank you very much in advance,
Carol