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Possible to run gradients using Shimadzu FCV-10AL mixer?

Posted: Sun Aug 05, 2018 3:37 pm
by lespaul
We're running isocratic runs on our Shimadzu HPLC (LC-10 VP) using one pump (LC-10ADVP) and a FCV-10AL mixer. I'm wondering if I could use this mixer to do gradient elution runs.

Reason I'm confused is because on the software (EZSTART 7.4) there is NO option to set mixture percentages (see screenshot). You can only set ONE channel (A,B,C,D) to choose from, not mix. To me, the FCV-10AL seems more like a switching valve than a gradient mixer (?).

BTW I do have another 2nd pump (LC-10ADVP) and a FCV-11AL that I can install if need be.

Unfortunately I cant find any sort of documentation from Shimadzu on how to set this up. Any input would be appreciated.

Screenshot: https://1drv.ms/u/s!AjglVmUkgtjLo29kuVxERqzLRhQW
FCV-10AL picture: https://1drv.ms/u/s!AjglVmUkgtjLo3JiqHUklMT36y4J
FCV-10AL: https://1drv.ms/b/s!AjglVmUkgtjLo3AAezq9Ov12Wwpz


Thanks again!

Re: Possible to run gradients using Shimadzu FCV-10AL mixer?

Posted: Mon Aug 06, 2018 5:39 pm
by hplcguruguy
set fcv type on pump front to fcv 10alvp, and gradient mode light will come on. reconfigure in class vp

Re: Possible to run gradients using Shimadzu FCV-10AL mixer?

Posted: Mon Aug 06, 2018 5:39 pm
by hplcguruguy
set fcv type on pump front to fcv 10alvp, and gradient mode light will come on. reconfigure in class vp

Re: Possible to run gradients using Shimadzu FCV-10AL mixer?

Posted: Thu Aug 09, 2018 7:05 pm
by lespaul
Thanks HPLCguru I tried this and now I see the gradient option. Thanks