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- Posts: 5
- Joined: Sat Jan 13, 2018 3:42 pm
I am doing a bioanalytical method validation. The biological matrix is liver microsomes and I normally prepare my calibration standards by spiking the analyte into a blank microsomes containing both Mgcl2, sodium pyrophosphate and potassium phosphate buffer. My sample prep is mostly protein precipitation using equal volume of cold acetonitrile.
I have discovered that whenever I run my batch of standards, the peaks are splitting. This is usually resolved after back flushing the column but I cannot continue doing this since it is taking up the analysis time.
I would appreciate any suggestion towards solving this problem.
Thank you.