"Calibration", as a term is not applied to an HPLC system. You can calibrate for a wavelength, but we do not calibrate HPLC systems at all. We "Qualify" them to see if they meet a specification (usually the manufacturer's); Example: An Instrument Qualification (IQ) or Operational Qualification (OQ). These check each of the component modules to see if they meet specific specifications established by the manufacturer. Pipettes
are calibrated. Balances
are calibrated. HPLC's
are not calibrated.
Lose terms such as Performance Verification (PV) usually are applied to an SOP which tests a specific metric, such as injection RSD or gradient composition. It can be almost anything that you properly define.
You Asked; "
What is the opinion on this topic - calibrating a hplc with or without column? Is there scientific advantage on this".
As mentioned, we do not calibrate an HPLC. We do run many tests though and most do NOT use a column (because in most cases, you are not trying the qualify the column, you are qualifying a specific module). Please refer to this short article, "
The HPLC Restriction Capillary; Troubleshooting, Qualification and Running Without A Column";
https://hplctips.blogspot.com/2018/04/t ... llary.html