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- Posts: 13
- Joined: Tue Apr 05, 2016 10:58 am
firs thanks so much for every one he try to help me . in my laboratory we have HPLC perkin elmer company . and i want separate food artificial colors ( tartrazine and allorared ) in juices and beverages . i tried but i got retention time early and poor resolution ( 1.91 for tartrazine standard and 2.32 for allora red standard ) -Column is C 18 , 250 mm , 4.6 mm . mobile phase was ( 10 % ACN : 90 % OPA 0.1 % ) . OPA = Orthophosforic Acid . and flow rate = 1.0 ml \ mint and run time 10 mint .
so please if any one can help me for this experiment ?
THANK YOU FRIENDS .