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- Posts: 392
- Joined: Thu Sep 08, 2005 4:12 am
- Location: Texas
I like to revisit: what happens if you inject a 5 uL sample in hexane to RP HPLC (C18 or C8, M.P. 10% to 95% ACN)?
Benefit: when hexane is used as extracting solvent and injected directly you do not need to worry about the potential loss of unknown during evaporation and reconstitution
The questions: what happen to hexane? stay in the column, bleeding, or eluted out completely every injection(eluted up to 95% ACN)?
how analytes behavior in column? polar, medium, and non-polar analytes, are they doing the same or different?
When it is OK and when not? why?