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- Posts: 28
- Joined: Mon Nov 07, 2011 9:05 am
I am quantifying ascorbic acid in pharmaceuticals with a 100% aqueous mobile phase, pH 2.6 adjusted with acetic acid.
During the first 5 injections of standard solution, peak area increases 4-fold, then stabilizes, which has no obvious explanation...
Can anyone help? Could this be a matter of column equilibration or dilution solvent?