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FITC Dextran analysis by HPLC

Posted: Wed May 09, 2018 1:37 pm
by jralic
Hi,
Has anybody any experience with FITC Dextran kD (HPLC/FLD)? What column to use, what mobile phase to use?
Thanks for your help

Ralic

Re: FITC Dextran analysis by HPLC

Posted: Wed Jun 27, 2018 3:44 am
by dap
Hello.
Sorry if offtopic. Dextrans can be analyzed via HPLC/RID & column like BioSep-SEC-S (Phenomenex).

Re: FITC Dextran analysis by HPLC

Posted: Thu Jun 28, 2018 9:32 am
by danko
Various Mw Dextran is actually used for Mw calibration in GPC/SEC analyses. Tosoh (TSK) columns are superb for purpous.
An example can be found here:

https://www.sigmaaldrich.com/content/da ... 413109.pdf

Best Regards