Analysis of Boronic Acid and Identified Boranes

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
I have a THF layer of Boronic acid. This is DIfluoro Boronic acid. I am analyzing it on HPLC. I have dissolve it in THF and made up with ACN and water combination up to 100ml. I have prepared it in 200 ppm level. Now the question is that we got incorrect AI as per synthesis team. I m not sure that how much they r true. But I want to know that is there is any specific method to analyze Difluoro Boronic acid. I also analyze it on GC with Pinacol derivatization but in bot of cases I did not found any unknown's rather than Boronic acid peak.

How can I get right analysis on HPLC for this difluoro Boronic Acid???
I m using Acquity BEH C-18 column, 100 x 2.1mm ID and 1.7µm
ACN : Water (0.085% H3PO4) : : 28 : 72
Wavelength : 210nm
Injection Qty : 1.0µl
I used to complex boric acid with tartaric, then RID, but I don´t know if that´s going to work for you.
Q. F. Ignacio Viera
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry