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- Posts: 35
- Joined: Sun Apr 08, 2018 3:17 am
I validated the dissolution procedure and cut the column down from a 250mm column to a 150mm column. At the time we got the required resolution (NLT 2) (Actual was something like 6). When we tried opening brand new columns, the peaks elute almost right on top of each other (Resolution 1.7)
Method Parameters:
Mobile phase: Monobasic potassium phosphate pH 2.35 containing 2mL of Triethylamine/L 92:8 Buffer Methanol.
Diluent/dissolution media: 0.01N HCl
Column: 250mm X 4.6mm, 5um L1 - USP column recommendation says Luna C18 - I tried running with a 150mm Luna C18(2) and with one column we got adequate resolution, and now with a brand new column we're having problems, both the USP and Phenomenex say that this is an appropriate column for Acetaminophen products.
Injection volume: 30uL
Column Temperature: ambient
Flow rate: 1.5mL/min
UV Detection: 214nm