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- Posts: 8
- Joined: Sat Mar 31, 2018 11:23 am
Suppose in a HPLC method, I have to take 1 g sample and during sample preparation it is diluted to 5000 times. my HPLC can quantify 1 ppm of that analyte in pure solvent (instrument LOQ) while 1.5 ppm in sample matrix.
Then during method LOQ determination, whether I have to incorporate all the dilutions or not? If I incorporate all the dilutions steps and inorder to meet my instrument LOQ( 1ppm), that sample should have at least 5000 ppm of that analyte. Is it means my method LOQ 5000 ppm??
or method loq just means incorporation of matrix only in loq determinmatrion ( 1.5 ppm in above example)
Hoping for much responses from the experts.
Thank you
Suraj