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- Posts: 36
- Joined: Tue Jun 10, 2008 6:49 pm
- Location: St. Louis
MPA: 20mM acetate in water and MPB: 100% ACN.
The gradient goes from 5% B to 70%B.
I'm using a low pressure mixing system without the inline degasser.
Mobile phases are thoroughly degassed.
I'm getting lots of air bubbles leaving my GPV, causing pressure changes, and low pressure.
I recently replaced the GPV with a refurbished one and this is still happening. So, I believe this is due to mixing.
From other discussions on this topic, I read that high pressure could resolve the bubbles because they are only produced at atmospheric conditions.
SO, I'm trying to figure out a fix. Here's what I'm thinking..
1) premix mobile phases
2) fix/replace degasser (agilent 1100)- would this help? Does it change the pressure of the lines?
3) replace some other pump part (already have new AIV, outlet ball valve, and seals.)
4) take a vacation and let someone else deal with it.
5) Buy a binary pump.
6) Add back pressure?
Since this has happened for other methods too, I'm hoping to have a good solution. I'm going to pre-mix today, but I would love to hear your thoughts or anything I haven't thought of.
Thanks!