by
varossf » Mon May 07, 2018 2:17 pm
lvan wrote:
We have recently purchased the new Chemstation Openlab software and a brand new Waters Alliance e2695 and the 2489 UV detector. My problem is that i ran a calibration curve and my peaks look good, but my area count is off by a factor of 10. For example, the area count for formaldehyde is 5.64 for level 1 in the cal curve (concentration is .0102 µg/mL.) Normally, my area count would be around 56.4 for level 1 with the same concentration.
Any ideas what is going on?
before anyone chimes in about why we did not by the waters software, it was the expense. We are a small lab.....it took me 15 years to get new software and equipment.....
Thanks for any help!!!
Dear,
Please tell us whcih system you have used in the past, the system to which you are comparing the Area to. You say that you have an area 10-fold smaller, maybe the cell length of the detector is shorter for a start? Maybe the settings of the previous detector where different like having an amplifier setting differently??
Just thinking loud out here.