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- Posts: 104
- Joined: Tue Aug 04, 2015 9:57 pm
I put in a call to the manufacturer and inquired as to how to possibly improve this as there is no gradient possibility in this analysis and the only MP that is documented as being suitable for this column is water.
They sent me one of their chromatograms and it had a K' situation that was nearly as bad with the lowest being 0.1. And then a somewhat humerous thing happened - I asked if perhaps a K' that low is acceptable in IEC and the response was to ask me for more details on my run conditions as they would like to help me improve that. And then I told them, no, I was talking about the K' for their run in their tech paper where t0 was based on an unretained marker compound.
And then I never heard back.
So, assuming a K' this low is equally unacceptable for ion exclusion chromatography as it is for RP chromatography, how would one go about improving it without a gradient option and being limited to pure water as a MP?