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- Posts: 47
- Joined: Fri Aug 05, 2011 6:47 pm
Sorry if the title of the post is a bit cryptic!
I'm in the process of developing a method that aims at separating 2 compounds by HPLC with UV-VIS detection. However neither of the 2 compounds displays profound UV activity, and sometimes I'm really sure whether the compounds have eluted or not.
Hence, I inject the reference sample with no column installed, and use that as a reference point with respect to the peak area, just to have a bearing with regards to the expected peak area.
But is there any pitfalls to this approach, and does any body else use this?
In advance thanks
Erik