By unknown on Sunday, July 11, 2004 - 06:46 am:

I wonder what is cause for negative peaks in LC using UV detection? Why these negative peaks disappear after changing (increasing) the temperature by few degrees?
On the other hand, while using a validated LC/UV method for several weeks, I suddenly have a very reproducible negative peak in the middle of the chromagram; that I didn't observe before with the same system. What can be the cause.