High Palcebo peaks in sample chromatogram

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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Hi all,
It has been quite long since my last post!!. I have a question,
In our drug product related substance chromatogram there are some peaks observed sample as well as in placebo. However, the peak response is higher in the sample (40 to 50% higher). Should we have to report it? Anybody faced such issues?. How to go ahead about that?

Appreciate valid responses,

That strongly implies that your sample contains peaks that co-elute with peaks from the placebo. I'm sorry to be the bearer of bad news, but at a minimum, that "excess" area should be reported. If it represents a sufficient area to be identified, I would recommend that you develop an orthogonal method to separate what is sample-related from the placebo.
-- Tom Jupille
LC Resources / Separation Science Associates
+ 1 (925) 297-5374
2 posts Page 1 of 1

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