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- Posts: 28
- Joined: Mon Nov 07, 2011 9:05 am
I have been trying to determine aromatic hydrocarbon types in middle distillates of petroleum products with HPLC-RID, heptane as mobile phase and an amino column, according to EN 12916.
However, after a few injections of the standard, my column exhibits poor retention and resolution for the 7 compounds. I replaced the column with a new one (different manufacturer) and obtained the same pattern.
System backpressure and baseline signal are stable. After receiving the column, I have equilibrated it directly with heptane for approximately 1 hour, and stored it in heptane.
Any suggestions concerning what may be happening and possible ways to troubleshoot?