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- Posts: 58
- Joined: Tue Oct 09, 2012 12:25 am
I know that I need to gradually increase the rate of my column flow until I get to my test method flow rate (i.e. increase rate of 0.2 ml/min until I reach my flow of 2.0 ml/min) to prevent column damage. Our SOP states to gradually decrease the flow back to 0 ml/min at a rate of 0.2 ml/min. I can see why we gradually increase the rate but I don't know why just stopping the flow to returning to 0 ml/min would cause fissures or some other sort of damage to the column. Can someone please explain? Thanks