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- Posts: 181
- Joined: Tue Oct 28, 2008 9:54 pm
- Location: usa
I am trying an assay/impurities method for a steroid. The graphite column shows great selectivity but the peak shape needs improvement. So far I have tried a simple gradient with 0.1%TFA and increasing ACN and got a bunch of shark fin peaks which look like mass overload. Not much was loaded though.
I also tried dilute H2SO4 with ACN gradient and H2SO4 with 0.3% SDS and ACN gradient and got poor tailing. Not shark fins but still bad.
I am trying positively charged surfactant next. Good or bad idea?
thanks!
Analytical Chemist and Adventurer
Venture Industries
Spider-Skull Island