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- Posts: 656
- Joined: Tue Jul 05, 2005 7:45 am
- Location: Denmark
I am running on a Kinetex HILIC 2.6 µm (3.0x150 mm), where I see a constant drop of plate count during the sequence. The sample is a peptide.
It runs with 10 mM ammonium acetate pH 5.2 in 90% acetonitrile
The samples are dissolved in mobile phase and filtered before injection and I see no issues with increasing pressure or drifting retention times. But the peaks are getting broader and broader...
According to the manual you should wash the column with 5% acetonitrile in 100 mM ammonium acetate pH 5.8. This I have done for two days, but I see no improvement at all!!
Does anyone have a good explanation what is going on, or a washing trick?