-
- Posts: 4
- Joined: Mon Jun 19, 2017 1:39 pm
I'm looking for a reliable method to monitor (and quantitate) the cleavage reaction of a 45 kDa protein by a protease. The cleaved fragment is 1 kDa long, so the targeted analytes should have 45, 44 and 1 kDa. The uncleaved protein is hydrophilic and stable in HEPES buffer for several weeks. The SDS-PAGE analysis of the reaction mixture is not really conclusive and it takes very long. Do you have any suggestions for a system capable of performing the separation on a normal PDA-HPLC?
Thanks in advance!