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By Ravi Ravichandran on Friday, July 16, 2004 - 08:35 am:
I am intersted in knowing if there would be any difference in using Tetrabutyl Ammonium hydroxide as a suppression agent vs tetramethyl ammonium hydroxide as a suppression agent.
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By Chris Pohl on Sunday, July 25, 2004 - 04:45 pm:
Ravi,
The deciding factors in choice of "suppression agent" are: extent of ionization, size, purity and cost. In the case of the two agents you mention, both are 100% ionized so this isn't a relevant factor. Both are available in high purity if you purchase it from Fluka or Sachem. TMAH is somewhat cheaper and as such might seem preferred but being much smaller suffers from substantially more leakage due to incomplete exclusion. TBAH will give a lower leakage background at 50mM than TMAH will at 10mM. In fact, TMAH isn't much better than KOH in terms of leakage. So, unless you are working with dilute eluents (less than 10mM), you will get significantly lower backgrounds and lower noise with TBAH.
I am intersted in knowing if there would be any difference in using Tetrabutyl Ammonium hydroxide as a suppression agent vs tetramethyl ammonium hydroxide as a suppression agent.
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By Chris Pohl on Sunday, July 25, 2004 - 04:45 pm:
Ravi,
The deciding factors in choice of "suppression agent" are: extent of ionization, size, purity and cost. In the case of the two agents you mention, both are 100% ionized so this isn't a relevant factor. Both are available in high purity if you purchase it from Fluka or Sachem. TMAH is somewhat cheaper and as such might seem preferred but being much smaller suffers from substantially more leakage due to incomplete exclusion. TBAH will give a lower leakage background at 50mM than TMAH will at 10mM. In fact, TMAH isn't much better than KOH in terms of leakage. So, unless you are working with dilute eluents (less than 10mM), you will get significantly lower backgrounds and lower noise with TBAH.
