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Problem with amino column!!! HELP
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
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Hi! I need guidance. I've started to use a waters spherisorb amino column, with the aim to analyze sugars. I activated the column in the conditions especified in the care manual, and then I started to flush several mobile phases through the column to conditioning it for sugar analysis. I used isopropanol/pentane, isopropanol, isopropanol/acetonitrile and finally acetonitrile. Each 20 min at 1mL/min. After equilibrating the system with the mobile phase I needed to determine sugars (ACN/H2O 80:20), I injected a sucrose standard and an inulin standard but both had the same retention time. It seems like the column is not interacting with the analytes. What can I do? How can I solve this?
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