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In this article, the BIOshell A400 Protein C4 column is shown to separate three smaller IgG fragments (Fd’, Fc, and the light chain) after the protease IdeS cleaves IgG. It resolves all three in minimal time with standard mobile phases for protein or peptide reversed-phase chromatography using elevated column temperature.
To view the full article PDF click on the link below:
http://goo.gl/LzvLyj
This article has been uploaded and made available by Separation Science.
Separation Science/Chromatography Forum/
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david.hills@sepscience.com