- admin
- Site Admin
-
- Posts: 647
- Joined: Wed Aug 11, 2004 12:19 am
By Dimitris Hatzinikolaou on Tuesday, March 23, 2004 - 03:56 am:
Hello,
This is an urgent inquiry, please.
Is it possible to use a LichrossorbNH2 column
to separate and quantify a sample that
has glucose, gluconic acid and fructose.
If yes at what approximate conditions??
Thanks to everyone in advance
-------------------------------------------------------------------------------------------------------
By Zelechonok on Tuesday, March 23, 2004 - 06:57 pm:
Most likely this will not work. Usually when sugars are analyzed on NH2 columns the mobile phase is ACN/water. Acidic compounds like gluconic acid will not elute from NH2 column with this mobile phase. If you add a buffer to the MP then sugars separation can be compromised.
-------------------------------------------------------------------------------------------------------
By Zelechonok on Thursday, March 25, 2004 - 06:41 am:
What is your detection system? MS, IR, UV, ELSD?