sugars [March 23, 2004]

By Dimitris Hatzinikolaou on Tuesday, March 23, 2004 - 03:56 am:

Hello,
This is an urgent inquiry, please.
Is it possible to use a LichrossorbNH2 column
to separate and quantify a sample that
has glucose, gluconic acid and fructose.
If yes at what approximate conditions??
Thanks to everyone in advance

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By Zelechonok on Tuesday, March 23, 2004 - 06:57 pm:

Most likely this will not work. Usually when sugars are analyzed on NH2 columns the mobile phase is ACN/water. Acidic compounds like gluconic acid will not elute from NH2 column with this mobile phase. If you add a buffer to the MP then sugars separation can be compromised.

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By Zelechonok on Thursday, March 25, 2004 - 06:41 am:

What is your detection system? MS, IR, UV, ELSD?