By ulm on Wednesday, December 10, 2003 - 04:00 am:

Hi

Can anybody help me to solve my problem:
How to quantify using HPLC with UV detector following compounds:
m-phenylene diamine,diaminoanisole and
2,4-diaminophenoxyethanol dihydrochloride

Many thanks

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By zelechonok on Wednesday, December 10, 2003 - 08:40 pm:

Take one of our Primesp columns (Primesep 200, 150 mm x 4.6 mm, for example) and analyze your mixture using MeCN/Water/TFA mobile phase. Keep TFA concentration 0.1% and adjust water/MeCN ratio until all peaks are resolved. The easiest way to do that is to add 0.1% TFA in each water and MeCN bottle.
Primesep columns specifically design to retain polar basic compounds that are not retaining by RP systems. Call us or e-mail if you have more questions. All information available on our website www.primesep.com