hi
I have problem

We just installed TSP FL 3000 Fluorescence Detector with Waters Binary Gradient (2 515 Pump with Mixer ) through SAT IN BOX to Empower II software . We injected Antharacin Std and Got Nice peak

Now we wants use this for Analysis Hydrogen Cynamide in Grape Fruit Extract
after Derivatisation .
We injected 20uL 10PPB STD Hydrogen Cynamide and Got Peak.
But when we injected real Sample of Grape Fruit We got PMT overflowed .
Now Last Two days we are trying to STD but we are unable to get PEAK

There is lots of Noise in Baseline and Humps

What could be problem ?

Is anybody has Method for Hydrogen Cynamide Screeing in Grapes ?
i