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- Posts: 406
- Joined: Wed Jul 18, 2012 6:45 am
- Location: Germany
Dears
This is a series of injections from the same vial: 10 µL (black), 8 µL (pink), 6 µL (green) and 5 µL (flat line in black).
I would expect the peak heights to correlate about linearly with injection volume, but this is far away from it. Any idea what could cause this?
Analytes are Acetophenone and Benzene, C18 column with ACN/H2O. Needle wash is acetonitrile and purge solvent is methanol/water 20/80.
Thanks a lot
Jörg
Update:
I turned the autosampler off and on again, now linearity is good. Fixed for the moment, but it is not nice to know that something like this can happen...
This is a series of injections from the same vial: 10 µL (black), 8 µL (pink), 6 µL (green) and 5 µL (flat line in black).
I would expect the peak heights to correlate about linearly with injection volume, but this is far away from it. Any idea what could cause this?
Analytes are Acetophenone and Benzene, C18 column with ACN/H2O. Needle wash is acetonitrile and purge solvent is methanol/water 20/80.
Thanks a lot
Jörg
Update:
I turned the autosampler off and on again, now linearity is good. Fixed for the moment, but it is not nice to know that something like this can happen...
