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- Joined: Thu Oct 13, 2011 4:34 pm
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The issue does not seem to be carryover, as there is nothing detectable in the blank injections immediately following any sample or standard injections. A brief summary of the method is outlined below.
EDTA derviatized with ferric nitrate nonahydrate solution
Buffer/Mobile Phase A is tetrabutyl ammonium bromide and sodium formate in water
Mobile Phase B is Methanol
EDTA elutes during 98% MPA and 2% MPB
The column is a Hypersil BDS C18 5 micron 4.6 x 250 mm
UV detection at 260 nm
Any insight would be greatly appreciated.