Re: Calibration curve by changing injection volume, validation?
Posted: Tue Apr 03, 2018 12:01 am
The same way you prove that your dilutions are precise: by examining the linearity of the calibration plot. In any case this should be done routinely as part of PQ ("Performance Qualification", for the uninitiated).At least this is how I would look at it from an auditor's perspective - how can you prove that your system is precise at all of the various injection volumes? That would be the biggest question to answer if you were to do it your way - in my opinion.
What it boils down to is: can you prepare your calibrators more accurately / precisely than the autosampler can make its injections?
By the way, I prefer to keep injection volume constant to minimize possible matrix effects, but either approach can be validated.