Best buffers to use at alkaline pH (> 8.0)

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

5 posts Page 1 of 1
Dear all,
Could anyone please suggest me good buffers to use at alkaline pH (> 8.0)
-- Tom Jupille
LC Resources / Separation Science Associates
+ 1 (925) 297-5374
Hi Saipraveen,

Excellent reference from Tom Jupille--of the basic buffers listed within I've used borate, tris and glycine as parts of mobile phases in QC work.
Ammonia, pKa 9.2, pH range 8.2 - 10.2, volatile, <10mM and <30°C is recommended with MS use
Gerhard Kratz,
This link has also a number of biological buffers that are suitable at alkaline pH, such as Tricine, Bicine and Ches. ... al-buffers
5 posts Page 1 of 1

Who is online

In total there are 12 users online :: 0 registered, 0 hidden and 12 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 12 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry