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Peaks separation
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
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what is the expected reasons for no separation of peaks of antibiotics on lc-msms all peaks almost appear close to each other
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Hi
If components have similar or very slightly different affinity for phases (mobile and stationary) then there will be almost no difference.
In case of LCMS e/m also comes into game.
Regards
If components have similar or very slightly different affinity for phases (mobile and stationary) then there will be almost no difference.
In case of LCMS e/m also comes into game.
Regards
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- Posts: 404
- Joined: Wed Nov 09, 2016 12:43 am
Try adding a 3rd solvent to your mobile phases, that is discriminatory. There are very few molecules that have a similar solubility in three solvents. Use a slow gradient ramp around these peaks.
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- Location: Illinois
What are your compounds? Usually antibiotics have a charge and mixed-mode chromatography is exploring small difference in ionic and hydrophobic properties of the molecule. In my 17 years using mixed-mode columns I only had one or two cases when compounds were not FULLY resolved. Let me know if I can help. Send me an email trough our website.
Vlad Orlovsky
HELIX Chromatography
My opinions might be bias, but I have about 1000 examples to support them. Check our website for new science and applications
www.helixchrom.com
HELIX Chromatography
My opinions might be bias, but I have about 1000 examples to support them. Check our website for new science and applications
www.helixchrom.com
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