Trometamol HPLC method

[gnikol21]

Hello to all,
Im searching for an HPLC method for determination of Trometamol in a drug product. Can anyone help me?
Thnx in advance

[Vlad Orlovsky]

Here is a method on Primesep 100 column. You will need ELSD, LC/MS or RI to monitor this:
http://www.sielc.com/Compound-TRIS.html

Contact me if you have questions.

[gnikol21]

Thnx for the reply Vlad. Do you have any experience with these methods? I have on my HPLC RI detector so i can use a column like Primesep 100. Both Primesep columns using ELSD for detection. Any idea what kind of detection is ELSD?

[Vlad Orlovsky]

we developed this method in the lab and tested it with Evaporative Light Scattering Detector. This can be monitored with RI. Limit of detection for ELSD is around 50 ppm. I don't know what you can reach with RI. In the method I mentioned only one column retains TRIS (Primesep 100).

[gnikol21]

I will try Primesep 100 and i hope our RI detector will be suitable for the detection of trometamol peak.
Thnx for the help Vlad

[lusse]

I will try Primesep 100 and i hope our RI detector will be suitable for the detection of trometamol peak.
Thnx for the help Vlad

Dear gnikol, what did you recive result with RI detector?

[Vlad Orlovsky]

Here is RI method for something very similar - 3 ethanolamines and morpholine:
https://helixchrom.com/compounds/morpholine/