by
w2695 » Thu Apr 26, 2012 2:53 pm
@ Hollow... Thank u...I havn't changed the check valves, wud definitely try dat. The flow stream is not constant. I did try collecting the Mobile phase after it passed thru detector and the volume was not 1 ml / min. It was more like 500-600ul.
@ Jack Silver.. Thank u . I dint realize that newly opened bottles hv particulates too.. I don't filter it.. guess I hv 2 start doing dat from now...
LC_labrat wrote:
Check Valves
Inline Filter
Solvent Frits
Did you change the check valves? What about the inline-filter? It tends to clog with buffers. Are you cleaning the system regularly with for example 70%Methanol 30%Water to remove the build up of buffer? Also you may want to change the solvent frits, especially for the buffer solvent line. If it is an isocratic method, you may want to try pre-mixing the mobile phase.
Thanks 4 d suggestions.. I havn't changed d check valves yet. I did try to declog the inlet filter in with 100% methanol and clean the system regularly with 50 : 50 MeOH : Water. I follow an isocratic method and I am using pre - mixed mobile phase. I will try changing the solvent frit for the mobile phase line and change the check valves.. Hope all ur suggestions work for me ..fingers crossed..