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- Posts: 656
- Joined: Tue Jul 05, 2005 7:45 am
- Location: Denmark
I need to develop a 2nd ID test for a peptide dissolved in water with 5% mannitol. Any good suggestions? 1st ID test is just retention time vs a standard.
I assume that diode-array spectra will be very hard to use since most related compounds have the same spectrum. Same with FT-IR, where also the water in the product will kill the signal?
MS is not an option since the CMO does not have it. Can optical rotation be selective enough?
Or is it that bad that TLC is the only way?