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- Posts: 4
- Joined: Fri Feb 10, 2012 10:23 am
I have a problem with my separation. The root of the problem is the guar gum contained in the sample, it apparently precipitates at the column when the organic solvent ratio increases during the gradient elution and gradually increases the backpressure at the column. I tried to flush the column with water afterwards but it does not seem to help. From my experience the guar seems to have some retention so it is not possible to pass it through the column by a short isocratic step at the beginning of the gradient, on the other hand it is not so slow to be precipitated and trapped on the precolumn.
I see two possibilities - to precipitate (or separate it in other way) during the preparation of the sample or to find a way how to dissolve it and get it out of the column at the end of sequence. Do you have any experience with the guar in this way? According to the literature it is insoluble in almost everything except water. However, except of this general statement I did not find any detailed solubility information source. I'm planning to try DMSO or DMF, do you have any other idea?
Thanks.