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- Posts: 2
- Joined: Wed Jan 11, 2012 11:20 am
sodium acetate trihydrate (pH 7.2) + TEA + TFA
acetonitrile+methanol+ sodium acetate trihydrate adjusted to pH 7.2(using glacial acetic acid) (10:10:5)
Derivatization using OPA and FMOC (online pre-column derivatization in the autosampler)
column: Zorbax Eclipse rapid resolution column (3.0mmx50mm, 1.8um)
Temp: 40C
Problems:
1) Frequent back pressure issues
2) varying areas when individual amino acids are injected
3) varying resolution times (run-to run) but relative positions of all 17 amino acids (in standard amino acid mix) remain the same.
(checked with the suppliers ...... no concrete solutions offered )
Any suggestions?????