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My sample concentration is very low and has lots of other fatty acids in the sample solution. I am not able to get UV response. Does anyone offer advice? Thanks.
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
wkhuang wrote:
. It also turned out that the inactive ingredients are not free fatty acids but medium chain triglycerides.
wkhuang wrote:
I'm developing an HPLC method for Lubiprostone assay and impurity method in a liquid dosage form.
My sample concentration is very low and has lots of other fatty acids in the sample solution. I am not able to get UV response. Does anyone offer advice? Thanks.
GaneshRAJ wrote:wkhuang wrote:
I'm developing an HPLC method for Lubiprostone assay and impurity method in a liquid dosage form.
My sample concentration is very low and has lots of other fatty acids in the sample solution. I am not able to get UV response. Does anyone offer advice? Thanks.
Hi wkhuang,
I had gone through your concern with respect to analytical method for assay and Related substance. I also finding the same problem so need to know whether it has resolved from your end regarding analytical method and if yes can u share some thoughts that will help me to work on as the sample concentration is very low and detection at low level with UV found to be more difficult.
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