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- Posts: 61
- Joined: Sat Nov 15, 2008 10:29 pm
- Location: Halifax, Nova Scotia
I'm running an Agilent 1100 with a G1315A DAD at 205 nm. Method uses 93:7:0.1 MeOH-H2O-TFA, 1 mL/min @ 60°C. Every so often (intermittent problem) this little bit of baseline noise comes up (11-12 minutes):
Sometimes the noise overlaps with analyte peaks. The peaks are present, which indicates that the detector is still working, but their shape is jagged rather than smooth. Also, I use the same instrument for a method with DAD at 260 nm, and I never see this type of noise.
My instincts tell me this could be related to the lamp (Sonntek 2000 hr bulb, not an Agilent lamp), or it could be an air bubble. I degas my mobile phase by sonicating under vacuum for a few minutes, and have a working G1322 degasser, but there is always room for improvement.
I welcome any and all feedback!