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- Posts: 92
- Joined: Tue Oct 30, 2007 2:17 am
We currently have an injection repeatability issue. Here are chromatographic conditions:
Injection size:5uL
Diluent:methanol
gradient
flow: 1mL/min
Column: C18 15cm*4.6mm*5um
mobile phases: MeOH with 0.05% TFA;H2O with 0.05% TFA
RSD of 5 injections of std can be upto 1.6%. And RSD for 5 injections of std varies from 0.1 to 1.6% for injection repeatability. This is tough for us to pass the spec of 2% RSD for sample analysis repeatability.
Please recommend typical contibuting factors to injection RSD.
Thanks