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- Posts: 22
- Joined: Wed Jun 09, 2010 8:36 am
I have problem with my HPLC (Agilent 1200 with quarternary pump).There is 4 channel, that i used for :
A : water
B : metanol
C : acetonitrile
D : other solvent (like phosphoric acid 0.1%)
But now the B channel of my MCGV can't perfectly close, because of bubble there.
When I did the gradient test,the result was failed.
I want to know :
what factor cause this problem?
Is there any human error in this case?
Do you have any experience like this case?
How about other HPLC merk (Waters,Shimadzu,perkin elmer,etc)?