Page 1 of 1
Waters 410 RI sinusoidal baseline problems
Posted: Wed May 19, 2010 1:31 pm
by project grizzly
I am using the 410 with a Hitachi D-7000 LC system. The 410 is exhibiting a sine wave in the baseline, with a period of about 2 hours. When I switch into purge, the sine wave has a higher amplitude and shorter period. Anyone seen something like this? Welcome to ideas.
Posted: Wed May 19, 2010 5:34 pm
by bhuvfe
The sine wave is generally common for RI and I have always thought that it was due to the heating cycles of the column oven. In your case it seems more the heating cycle of your RI (at what T are you using it?).
Maybe you can switch off the column oven and see what happens?
If the temperature of your lab doesn't change extremely fast you should get a drifting baseline (either upwards or downwards) after a while..
Normally the sine baseline is not so critical if the signal is high enough and the amplitude is reasonable (at least considering I use RI only for GPC).
Re: Waters 410 RI sinusoidal baseline problems
Posted: Fri May 11, 2018 3:12 am
by rikky7777
Looks like a Temperaure fluctuation, what temperatures are you using in your column oven and inside temperature of your Waters 410?
Re: Waters 410 RI sinusoidal baseline problems
Posted: Fri May 11, 2018 3:36 pm
by Multidimensional
That sounds like temperature fluctuation for sure. Here is an article link that will help you understand and troubleshoot the problem.
"
HPLC Baseline Stabilization Tips for Refractive Index Detectors (RI or RID)" ;
https://hplctips.blogspot.com/2018/02/hplc-baseline-stabilization-tips-for.html