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- Joined: Mon Dec 03, 2018 11:10 am
Column: kromasil C18 250*4.6mm 5u
Mobile Phase : 100% Methanol
Flow Rate : 1ml/min
Detector: uv at 254nm
Diluent : Mobile phase
Try this method if you r using pure form of D3. If it is in matrix form heat the sample with mobile phase for 5-10min.