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- Posts: 1
- Joined: Tue Jun 20, 2023 10:12 pm
- Location: usa
I start the gradient a few percent below the amount I think my target will elute, and have the method set to increase by 15% in case I'm a bit off. If it elutes right on time, id like to up the solvent to my wash concentration so I don't have to sit through the entire gradient, while still being able to collect and plot what comes off in the wash.