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- Posts: 118
- Joined: Thu Jan 07, 2021 6:45 pm
I've recently attached a Prep column to our semi-prep system and was wondering about the responses I've been getting for samples that have been run. Since the flow-rate differs wildly (1 ml/min vs 20 ml/min), I was wondering how this should affect my detection since I have not been getting the usual responses.
On my analytical column, I see responses that frequently blow-out my PDA at specific wavelenghts (254, 320 nm), while the same volume injection on my Prep barely has any response. Is this due to the flow-rate differential between the two methods and, thusly, less-signal because my sample spends less time within the PDA? Or is this an issue that may be related to one of my columns?
Thank you.