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- Posts: 8
- Joined: Thu Feb 09, 2023 10:23 am
- Location: Newcastle upon Tyne, United Kingdom
Please can someone explain the differences in the chemistry between these two HPLC columns:
Agilent ZORBAX RRHD Eclipse Plus C18 (50 mm x 2.1 mm, 1.8µm)
Waters Acquity UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 μm)
I'm getting very little retention with the ZORBAX, even with 100% aqueous isocratic flow. I've seen some reports of others having success with the Acquity column, for the same compound as I'm developing a method for. I just want to understand the differences between these two columns before I buy one. Thank you.
https://www.ncl.ac.uk/cancer/people/pro ... stley.html